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cd41 cd61 pe  (Miltenyi Biotec)


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    Miltenyi Biotec cd41 cd61 pe
    Cd41 Cd61 Pe, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 90/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/cd41 cd61 pe/product/Miltenyi Biotec
    Average 90 stars, based on 2 article reviews
    cd41 cd61 pe - by Bioz Stars, 2026-02
    90/100 stars

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    Antibodies with the conjugated fluorophore, its isotype, and the concentration used after antibody titration.

    Journal: Animals : an Open Access Journal from MDPI

    Article Title: Chicken Immune Cell Assay to Model Adaptive Immune Responses In Vitro

    doi: 10.3390/ani11123600

    Figure Lengend Snippet: Antibodies with the conjugated fluorophore, its isotype, and the concentration used after antibody titration.

    Article Snippet: In this study, immune cells were stained with mouse anti-chicken CD3-Allophycocyanin (APC) (CT-3, SouthernBiotech, Birmingham, AL, USA), CD4-Spectral Red (SPRD) (CT-4, SouthernBiotech, Birmingham, AL, USA), CD28-Phycoerythrin (PE) (AV7, SouthernBiotech, Birmingham, AL, USA), CD8-APC (CT-8, ThermoFisher Scientific, Waltham, MA, USA), CD45-Fluorescein isothiocyanate (FITC) (LT40, ThermoFisher Scientific, Waltham, MA, USA), CD41/CD61-(R)PE (11C3, ThermoFisher Scientific, Waltham, MA, USA), and human anti-chicken CD25-FITC (AbD13504, Bio-Rad Laboratories, Hercules, CA, USA) antibodies.

    Techniques: Concentration Assay, Titration

    (A) K562 cells were incubated with 5 nM PMA for the indicated time and then the expression of CD41 and CD61 were analyzed by flow cytometry. (B) Cell morphology was observed by microscope. Scale bars represent 50 µm. (C) Megakaryocytic differentiation was detected by modified Wright-Giemsa staining for cell morphology. Representative cytological changes at 72 h, such as increase in nuclear-to-cytoplasm ratio (a), larger cells (b), and polylobulation nucleus (c) were denoted. Scale bars represent 50 µm. (D) K562 cells were induced with different concentration PMA for 72 h. Cell apoptosis were detected by flow cytometry. All graphics represented means ± SD obtained from three independent experiments, * p ≤0.05, ** p ≤0.001.

    Journal: PLoS ONE

    Article Title: Megakaryocytic Differentiation of K562 Cells Induced by PMA Reduced the Activity of Respiratory Chain Complex IV

    doi: 10.1371/journal.pone.0096246

    Figure Lengend Snippet: (A) K562 cells were incubated with 5 nM PMA for the indicated time and then the expression of CD41 and CD61 were analyzed by flow cytometry. (B) Cell morphology was observed by microscope. Scale bars represent 50 µm. (C) Megakaryocytic differentiation was detected by modified Wright-Giemsa staining for cell morphology. Representative cytological changes at 72 h, such as increase in nuclear-to-cytoplasm ratio (a), larger cells (b), and polylobulation nucleus (c) were denoted. Scale bars represent 50 µm. (D) K562 cells were induced with different concentration PMA for 72 h. Cell apoptosis were detected by flow cytometry. All graphics represented means ± SD obtained from three independent experiments, * p ≤0.05, ** p ≤0.001.

    Article Snippet: Cell differentiation was evaluated by staining of megakaryocytic lineage marker CD41 and CD61 (eBioscience) conjugated with phycoerythrin (PE).

    Techniques: Incubation, Expressing, Flow Cytometry, Microscopy, Modification, Staining, Concentration Assay

    (A) K562 cells were pre-treated with different doses of SA for 3 hour, and then the mitochondrial membrane potential (JC-1 staining) was determined by flow cytometry analysis. (B) K562 cells (3×10 5 cells/ml) were pre-treated with 5 µM CsA for 6 h and then treated with 5 nM PMA for the indicated time. The mitochondrial protein complexes were separated by hrCN-PAGE gel and then identified by in-gel catalytic activity assay of complex IV. (C & D) K562 cells were pre-treated with the complex IV specific inhibitor SA for 3 h, then cells were induced by 5 nM PMA for 72 h. Expression of CD41 and CD61 was determined by flow cytometry analysis. Data were shown as mean ± SD of three independent experiments, * p ≤0.05, ** p ≤0.001.

    Journal: PLoS ONE

    Article Title: Megakaryocytic Differentiation of K562 Cells Induced by PMA Reduced the Activity of Respiratory Chain Complex IV

    doi: 10.1371/journal.pone.0096246

    Figure Lengend Snippet: (A) K562 cells were pre-treated with different doses of SA for 3 hour, and then the mitochondrial membrane potential (JC-1 staining) was determined by flow cytometry analysis. (B) K562 cells (3×10 5 cells/ml) were pre-treated with 5 µM CsA for 6 h and then treated with 5 nM PMA for the indicated time. The mitochondrial protein complexes were separated by hrCN-PAGE gel and then identified by in-gel catalytic activity assay of complex IV. (C & D) K562 cells were pre-treated with the complex IV specific inhibitor SA for 3 h, then cells were induced by 5 nM PMA for 72 h. Expression of CD41 and CD61 was determined by flow cytometry analysis. Data were shown as mean ± SD of three independent experiments, * p ≤0.05, ** p ≤0.001.

    Article Snippet: Cell differentiation was evaluated by staining of megakaryocytic lineage marker CD41 and CD61 (eBioscience) conjugated with phycoerythrin (PE).

    Techniques: Membrane, Staining, Flow Cytometry, Activity Assay, Expressing